Ashvani Kumar Chaudhari*, Shiv Kumar Dwivedi*
Department of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow – 226025, India
ARTICLE INFOR: Received: 03 May 2023; Revised: 15 May 2023; Accepted: 16 May 2023
Cite this article: Chaudhari, A.K., Dwivedi, S.K., 2023. Biofabrication and Characterization of Silver Nanoparticles using biomolecules of Cassia fistula Flower. J. Appl. Sci. Innov. Technol. 2 (1), 38-42.
- Biofabrication refers to the process of creating nanoparticles using biomolecules.
- Cassia fistula flower biomolecules enable sustainable AgNPs synthesis.
- Analysis optimizes silver nanoparticles for diverse applications.
In the last few years, the application of biological agents, like plant extracts, for the biological synthesis of nanoparticles (NPs) has become increasingly popular. This research focuses on the biosynthesis of silver NPs with the help of extract from flower of Cassia fistula (Cf). The study investigated several parameters, including AgNPs concentration (ranging from 1 to 100 mM), aqueous extract (100 to 900 µL), pH (4 to 10), incubation time (15 to 119 min), and temperature (30°C- 90°C) to determine their effect on the synthesis of AgNPs. The optimal conditions for synthesis were found to be a 100 mM concentration of AgCl2, a temperature of 30 °C, a pH of 6.0, and duration of 120 min. The NPs were characterized by UV-Visible spectroscopy, which indicated an absorption peak at around 462 nm. X-ray diffraction (XRD) revealed that the synthesized nanoparticles showed a crystalline structure with a size extending from 78 to 120 nm, and SEM analysis demonstrated that they were nearly spherical in shape with no agglomeration.
Keywords: AgNPs; SEM; XRD; FTIR; Flower extract
Scope: Environmental Engineering & Sciences